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. Author manuscript; available in PMC: 2019 Oct 22.
Published in final edited form as: Nat Microbiol. 2019 Apr 22;4(7):1173–1182. doi: 10.1038/s41564-019-0427-4

Figure 3. Dot/Icm-dependent polar targeting of the Legionella core-transmembrane subcomplex.

Figure 3.

A) Broth grown L. pneumophila cells were probed with primary antibodies (polyclonal for DotH, DotG, and DotF or HA monoclonal for DotD-HA and DotC-HA), decorated with secondary antibody conjugated with Oregon green and imaged with fluorescence microscopy. Samples assayed include the wild-type strain Lp02 (WT), a strain lacking all 27 dot/icm genes (SΔ), and the SΔ strain expressing individual core-transmembrane subcomplex components. B) DotH localization in wild-type Legionella and in individual dot/icm deletions. DotH was detected by immunofluorescence microscopy in wild-type cells (WT) and individual dot/icm mutant strains. The corresponding deletion strain is boxed in yellow and dot/icm deletions that are affected are boxed in red. Representative images are shown from three independent experiments. Scale bar 2 μm (A,B).