FIGURE 5.

FACS analysis of inflammatory cell populations within doxycycline treated wounds over time. A, FACS gating strategy for the MAC1+, B220+, and double negative populations. B, Effects of doxycycline on relative populations of macrophage (MAC1+), B cell (B220+), and lymphocyte (double negative, Lymph) cell populations in the wounds at 12 hours (top left panel), 5 (top right), 9 (bottom left), and 14 days (bottom right) postinjury. Macrophages are significantly increased with doxycycline treatment at day 9 postinjury (n = 6 wounds, *P = 0.0407). *, denotes statistical significance. Results depicted as mean per animal ± SEM.