Figure 10. Immunocytochemical analysis of the effects of systemic delivery of the siα-syn conjugated to C2-9r or ApoB¹¹ peptides on microgliosis in the brains of α-syn tg mice.

Four-month-old α-syn tg and non-tg mice received repeated intraperitoneal injections of scrambled siRNA (si-sc) or α-syn siRNA (si-αsyn) conjugated to either C2-9r or ApoB¹¹ and were sacrificed 4 weeks later for analysis. Sections were immunolabeled with an antibody against the microglia cell marker-Iba1. (A) Representative images of the neocortex, hippocampus and striatum of non-tg and α-syn tg mice treated with either si-sc or si-αsyn conjugated with C2-9r or ApoB¹¹ of sections immunostained with the Iba1 antibody. Image analysis of the relative numbers of Iba1 immunoreactive cells per 10² in the (B) neocortex, (C) hippocampus (CA3), and (D) striatum. One-way ANOVA with poshoc Dunnet when comparing to control and Tukey-Kramer when comparing in between groups. * indicates statistical significance p < 0.05 compared to non-tg mice. ** p < 0.05 compared to C2-9r/si-sc. # indicates statistical significance p < 0.05 compared to α-syn-tg mice treated with ApoB¹¹/si-sc. Scale bar represents 250 μm for low magnification panels and 25 μm for higher magnification panels. Non-tg (n=16) and α-syn tg (n=24).