Figure 5. Immunocytochemical analysis of the effects of systemic delivery of the siα-syn conjugated to C2-9r or ApoB¹¹ peptides on accumulation of α-syn in transgenic mice.

Four-month-old α-syn tg and non-tg mice received repeated intraperitoneal injections of scrambled siRNA (si-sc) or α-syn siRNA (si-αsyn) conjugated to either C2-9r, ApoB¹¹ and were sacrificed 4 weeks later for analysis. Sections were immunolabled with an antibody against FL-α-syn and analyzed by bright field microscopy. (A) Representative images of the neocortex, hippocampus and striatum of non-tg and α-syn tg mice treated with either si-sc or si-αsyn conjugated with C2-9r or ApoB¹¹. Image analysis of levels of expressed as corrected optical density for α-syn immunoreactivity in the (B) neocortex, (C) hippocampus (CA3), and (D) striatum. One-way ANOVA with poshoc Dunnet when comparing to control and Tukey-Kramer when comparing among groups. Statistical significance p < 0.05 compared to: * non-tg mice; ** C2-9r/si-sc; # ApoB¹¹/si-sc. Non-tg (n=16) and α-syn tg (n=24). Scale bar represents 250 μm for low magnification panels and 25 μm for higher magnification panels.