Fig. 5.

Omentectomy or sham operation modified the peritoneal cellular composition. Male CD-1 mice (n=4 per group) were subjected to omentectomy or sham operation, and peritoneal cells were obtained by lavage of the peritoneum at 1, 14 and 28 days post-surgery. Cells were centrifuged for 10 min at 300xg, resuspended in PBS without Ca²⁺/Mg²⁺ supplemented with 0.5% BSA and counted. A group of non-treated mice was also used as a control. Cells were stained as described in the Materials and Methods section, and flow cytometry was performed using a FACSCanto II flow cytometer with FACSDiva software. The data were analyzed using FlowJo software v.10.1. Representative Ly6G/F4/80 density plots of CD19⁻CD11b⁺ gated viable cells are shown for peritoneal cells harvested at 1 day (A), 14 days (B) or 28 days (C) post omentectomy or sham surgery.