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. 2019 Jun 21;3(2):026105. doi: 10.1063/1.5090986

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© Author(s).

2473-2877/2019/3(2)/026105/11

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FIG. 5. — Analysis of the flow profile. (a) Widefield image of the perfused lumen. Boxes are showing the predetermined regions of interest (ROI) at 2.5 mm, 5 mm, and 7.5 mm from the inlet. The average diameter of ROI 1: 320 μm, ROI 2: 290 μm, and ROI 3: 300 μm. A full impression of the perfused lumen is presented in video S3. (b) Velocity profile reconstructed of 30 frames per ROI showing maximum velocity in the center of the lumen. Some interaction of the beads with the cell wall can be observed. Using maximum velocity and assuming laminar flow the volumetric flow rate is determined to be, respectively, 19 μm/min, 18 μm/min, and 18 μm/min. (c) Calculated shear stress per ROI using the determined flow rates and measure diameters. ROI 1: 0.24 Pa, ROI 2: 0.30 Pa, and ROI 0.28 Pa. Scale bar: 1000 μm.