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. 2019 Jun 21;10:2727. doi: 10.1038/s41467-019-10583-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Plasma IdeS pre-treatment in AP–DIA. a Cluster analysis based on z-score of the M1 protein AP–DIA data in human plasma and IdeS pre-treated human plasma. sfGFP was used as a negative control. b Plasma IdeS cleavage counteracts IgG-mediated C1Q attachment to the M1 protein. c Effect of plasma IdeS pre-treatment on complement C1Q, C1R, and C1S binding to the M1 protein. Error bars are expressed as standard deviation (s.d.) from the mean. All experiments were prepared using n = 3 biologically independent samples. Source data are provided as a Source Data file