Fig. 2.

Stepwise activation of TRFS-green by TCEP. a TRFS-green (20 μM) was incubated with TCEP (1 mM) in TE buffer (50 mM Tris-HCl, 1mM EDTA, pH 7.4) at 37 °C for 24 h. The reaction mixture was analyzed by HPLC, and the quantification of intermediate and ANA was shown in (b). c Time-dependent fluorescence changes of TRFS-green (10 μM) in the presence of TCEP (1 mM) in TE buffer at 37 °C. The inset shows the time-dependent changes of emission at 538 nm (λ_ex = 438 nm). Source data are provided as a Source Data file.

Source Data file