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. 2019 Jun 21;9:9027. doi: 10.1038/s41598-019-45195-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mouse iLEC characterization. (a) Characterization of iLEC by flow cytometry show a small fraction ~15% of the epithelial cells express lung transcription factor Nkx2-1. (b) Immunofluorescence characterization of mouse Cd326+ iLECs. Scale bar represents 45 μm. White arrows point to positive cells. (c) Unsupervised hierarchical clustering shows global gene expression of mouse iLEC distinct from adult and embryonic lung cells and some distinct expressions compared to parental MEF. (d) Principal component analysis shows mouse iLEC segregated from all three control cell types (adult and embryonic lung epithelial cells and parental MEF). (e) Principal component analysis of single cells from iLEC, Cd326+ adult lung cells and mcherry-expressing embryonic (e18.5) lung cells (Blue dots represents e18.5 lung cells, red dots represent adult lung cells and green dots represent iLEC). (f) Heatmap of the lung genes expressed in 6 single iLEC that clustered with control embryonic mcherry+ and adult CD326+ lung epithelial cells. Green asterisk mark the column for each iLEC.