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. 2019 Jun 21;10:2735. doi: 10.1038/s41467-019-10676-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Tumor burden is reduced in St2 receptor-deficient gp130^FF mice. a Quantification of total tumor burden in 100-day-old mice of the indicated genotype. Each symbol represents an individual mouse. One-way ANOVA was performed with F (DFn, Dfd) = 11.83 (2, 48). b Enumeration of total tumor number from mice in a, and of tumors following classification according to their size. n = 12 (FF, St2^+/+), n = 20 (FF, St2^+/−), and n = 19 (FF, St2^−/−) mice. One-way ANOVA was performed with F (DFn, Dfd) = 22.79 (11, 192). c Quantification of toluidine blue (for detection of mast cells; submucosal tissue), F4/80 and CD31 stained sections of gastric tumors of mice of the indicated genotype. Mast cells: n = 10 mice, t-test t (df) = 4.25 (18); F4/80: n = 8 (FF; St2^+/+), n = 9 (FF; St2⁻^/−), one-way ANOVA F (DFn, Dfd) = 27.52 (3,29); CD31: n = 6 (Submucosa) n = 5 (Tumor), and one-way ANOVA F (DFn, Dfd) = 13.6 (3,19). d qPCR expression analysis of chemokines expressed by FACS-purified tumor-associated mast cells from stomachs of either FF; St2^+/+ or FF; St2^−/− mice. All n = 4 from two independent experiments. Csf2: t-test t (df) = 3.81 (6); Ccl3: t-test t (df) = 3.97 (6); Ccl7: t-test t (df) = 0.88(6); Il6:: t-test t (df) = 4.02 (6); e, f Flow cytometric analysis of unaffected antrum (AN) and antrum tumors (AT) of indicated genotype for the frequency of ILC2 cells (lineage⁻, Cd11b⁻, Gata3⁺), Tregs (Foxp3⁺, CD4⁺), and proportion of St2⁺ cells within these cell types. FF; St2^+/+ n = 7 and FF; St2^−/− n = 6, from two independent experiments. ST2⁺/ILC2: t-test t (df) = 4.39 (12); ST2⁺/Treg: t-test t (df) = 0.98 (12). g Enumeration of total tumor burden at 14 weeks of age of FF; St2^−/− host mice, which received tail vein injections of either FF; St2^−/− or FF, St2^+/+ bone marrow-derived mast cells (BMMC) (n = 8 mice per group). Mann–Whitney test was performed with Mann–Whitney U = 11.5. Data are represented as mean ± SEM, with p values p < 0.05 considered being significant. Source data are provided as a Source Data file. See also related Supplementary Fig. 6