Fig. 5.

Wild-type IncA rescues the activity of non-fusogenic IncA when expressed in trans. a HeLa cells were co-infected with the indicated IncA_mutant-FLAG complemented IncA KO strains (IncA mutant, green) at a MOI of 5 and wild-type C. trachomatis expressing mCherry (L2_mCherry, red) at a MOI of 2. IncA_mutant-FLAG expression was induced with anhydrotetracycline at 5 hpi. Not induced (DMSO) served as a negative IncA KO control. Cells were fixed at 24 hpi and DNA was stained with Hoechst (gray). L2_mCherry (red) and IncA_mutant (green) inclusions that have undergone fusion contain both red and green Chlamydia. Scale bar = 10 µm. b Quantification of heterotypic fusion between L2_mCherry and IncA_mutant inclusions. Data were normalized to IncA_WT-FLAG. Graphs display the means of three independent experiments ± the standard deviation. Asterisks (***) denote a p-value < 0.001 (two-tailed student t-test). Source data are provided as a Source Data file