Fig. 5.

Mutation histories inferred from the bulk and single-cell sequencing data for a patient with childhood ALL (Patient 1 of the study in ref. ³⁷): a Clonal trees compatible with the bulk-sequencing data (inferred with CTPsingle²⁰). Clones are annotated with the mean VAF of the mutations newly acquired by the clone. b Mutation tree inferred with SCITE²⁸ from the single-cell panel sequencing data of 111 cells. The colouring scheme follows from the clustering in the CTPsingle trees. Mutations are annotated with the VAFs observed in the bulk sample. c Mutation tree inferred with B-SCITE from the combined single-cell and bulk data. B-SCITE infers the same early branching event as SCITE, but finds a mutation ordering that is in better congruence with the bulk VAFs. B-SCITE mutation trees can be compressed to clonal trees (Supplementary Fig. 29)