Fig. 7.

Mutation histories inferred from bulk (targeted sequencing with the average depth of coverage ∼100,000×) and single-cell (16 cells) data for a patient with triple-negative breast cancer from ref. ³⁸: a clonal tree implied by hierarchical clustering from the original study³⁸. Clustering was performed based on the profiles of several hundreds of mutations and four populations of cancerous cells were detected. In the same study, a subset of mutations was selected from each cell population and each of them is listed in the label of the corresponding population. b Mutation tree inferred with SCITE²⁸. The colouring scheme follows the colouring used in the clonal tree. Mutations are annotated with the VAFs observed in the bulk sample. c Mutation tree inferred with B-SCITE from the combined single-cell and bulk data. B-SCITE infers highly similar branchings as in the clonal tree and also finds a mutation ordering that is in congruence with the bulk VAFs. B-SCITE mutation trees can be compressed to clonal trees (Supplementary Fig. 29)