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. 2019 Jun 21;9:9057. doi: 10.1038/s41598-019-45510-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The proportion of RGCs with neurite extensions (%) 3 days after applying a hydrostatic pressure of 0 mmHg (n = 22 devices, n_0,RGCs = 993), 10 mmHg (n = 4 devices, n_0,RGCs = 106), 20 mmHg (n = 6 devices, n_0,RGCs = 534), 25 mmHg (n = 7 devices, n_0,RGCs = 503), 30 mmHg (n = 11 devices, n_0,RGCs = 552), 40 mmHg (n = 9 devices, n_0,RGCs = 370), and 50 mmHg (n = 11 devices, n_0,RGCs = 432 RGCs) for 72 hours. Data are in mean ± SEM. *, # and + indicate statistically different results from 0 mmHg (P < 0.05), 10 mmHg (P < 0.05), and 20 mmHg (P < 0.05) respectively by one-way ANOVA followed by Bonferroni post-hoc test. N_0,RGCs represents the number of RGCs with neurite extensions at the baseline.