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. 2019 Jun 21;9:9057. doi: 10.1038/s41598-019-45510-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Sholl analysis of primary RGCs, (c–h) Box chart of the relative number of intersections between neurites and concentric circles(R = 19.5 µm, 26.0 µm, 32.5 µm, 39.0 µm, 45.5 µm and 52.0 µm)after applied hydrostatic pressures of 0 mmHg (n = 155 RGCs), 10 mmHg (n = 67 RGCs), 20 mmHg (n = 109 RGCs), 25 mmHg (n = 77 RGCs), 30 mmHg (n = 76 RGCs), 40 mmHg (n = 80 RGCs), and 50 mmHg (n = 74 RGCs) on day 3. Box plot whiskers indicate the maximum and minimum values of the data. The symbols *, # and + indicate statistical significance relative to 0 mmHg (P < 0.05), 10 mmHg (P < 0.05), and 20 mmHg (P < 0.05), respectively, using One-way ANOVA followed by Bonferroni Post-hoc Test.