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. 2019 Jun 21;9:9057. doi: 10.1038/s41598-019-45510-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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A step-by-step schematic diagram of the production of PDMS devices: (a) laser cutting of the PMMA board into the desired trench pattern, (b,c) fabrication of PMMA mold, (c) replication of trench pattern onto PDMS, (d) peeling of PDMS chamber from pattern, (e) fabrication of plain PDMS with inlet and outlet, (f) bonding of two pieces of PDMS by oxygen plasma, and (g) bonding of PDMS device to glass slide by oxygen plasma.