Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 21;2:238. doi: 10.1038/s42003-019-0468-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — GhTGA3 activation activity and GhBOP1 promotion potential. a EMSA analysis of the specific binding of GhTGA3 to the probe containing the TGACG motif. GhBOP1 proteins were incubated with the biotin-labelled DNA probe in the reaction mixture for 30 min. The indicated amounts of unlabelled probe were used in the competition assay. The TGACG motif sequences are highlighted in red. b EMSA analysis of the efficiency of GhBOP1 in the binding activity of GhTGA3 to the TGACG motif. GhTGA3 proteins were incubated with the biotin-labelled probe, which contain two TGACG motifs in the present of GhBOP1. c Dual-luciferase reporter assay of the activation of GhTGA3 in Arabidopsis protoplasts. The effector vectors pRT-BD and pRT-BD-VP16 were used as negative and positive controls, respectively. Error bars represent the SD of three biological replicates. Each sample consisted of three technical repeats. Double asterisks indicate statistically significant differences, as determined by the Student’s t-test (**P < 0.01)