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. 2019 Jun 21;2:238. doi: 10.1038/s42003-019-0468-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — GhBOP1 enhances GhTGA3 activating activity in plants. a GUS staining analysis of GhBOP1 enhances GhTGA3 transcriptional activation to the GhPR1 promoter in tobacco leaf at 48 h after Agrobacterium infiltration with the indicated vectors. b Quantitative analysis of GUS activities in a. Error bars represent the SD (n = 18) of three biological replicates. Asterisks indicate statistically significant differences, as determined by Student’s t-test (**P < 0.01). c GUS staining analysis of GhBOP1 enhancing GhTGA3 transcriptional activation to the GhPR1 promoter in WT and transgenic cotton cotyledon at 48 h after Agrobacterium infiltration with GhPR1pro:GUS or GhPR1pro:GUS and GhTGA3-RNAi, respectively. CBB (Coomassie brilliant blue) was used to normalize the protein extracted from tobacco leaves. The values indicate the relative blue densities of corresponding wells tested by OD450, the blue density of WT was normalized as 1. d GUS activity analysis in c. Error bars represent the SD (n = 18) of three biological replicates. Asterisks indicate statistically significant differences compared to WT, as determined by Student’s t-test (*P < 0.05, **P < 0.01)