Table 1.
Primers used in this study
| Gene name | Purpose | Forward primer | Reverse primer |
|---|---|---|---|
| GhBOP1 | pHANNIBAL-1; RNAi | CGGGAATTCCAGAAACAAACCACAGCACTCC | CGGGGTACCTAGGGTTGTCTTTCACGAGTAAA |
| GhBOP1 | pHANNIBAL-2; RNAi | CGGTCTAGACAGAAACAAACCACAGCACTCC | CGCGGATCCTAGGGTTGTCTTTCACGAGTAAA |
| GhBOP1 | pBin438; OE | CGCGGATCCATGAGTAGCTTTGAGGAGTCCTTAAG | ACGCGTCGACCTAGAAATCATGAGAGTGATGGTACATT |
| ProGhBOP1 | pBIN121 | CCCAAGCTTTAGGCTTCACTTTGGCTTCGAT | CGCGGATCCTTTAGGGTTGTTTTTCACGAGAAAAAG |
| GhBOP1 | pGBKT7 | CGGGAATTCATGAGTAGCTTTGAGGAGTCCTTAAG | CGCGGATCCCTAGAAATCATGAGAGTGATGGTACATT |
| GhTGA1 | pGADT7 | CGGGAATTCATGGCGACCCAACACTTGG | CGCGGATCCCTAAGGCAAGGAAGGTTCAGGA |
| GhTGA2 | pGADT7 | CGGGAATTCATGGGCAGTAGAACGTTGAAAAAT | CGCGGATCCTCACTCGCGAGGTCTAGCCAG |
| GhTGA3 | pGADT7 | CGGGAATTCATGACAATATACGAGCAACTAAACC | CGCGGATCCTCAGAGTGAACTGAGAGCTCGG |
| GhTGA4 | pGADT7 | CGGGAATTCATGGCTAATGGGCTCATACTCCT | CGCGGATCCTTATGCAGGTTCACGAGCACAAG |
| GhTGA5 | pGADT7 | CGGGAATTCATGCCGGGTTTTGACTCACA | CGCGGATCCTCACTCTCGGGGTCGGGC |
| GhBOP1 | pCambia-NLuc | CGCGGATCCATGAGTAGCTTTGAGGAGTCCTTAAG | ACGCGTCGACCTAGAAATCATGAGAGTGATGGTACATT |
| GhTGA1 | pCambia-CLuc | CGGGGTACCATGGCGACCCAACACTTGG | ACGCGTCGACCTAAGGCAAGGAAGGTTCAGGA |
| GhTGA2 | pCambia-CLuc | CGGGGTACCATGGGCAGTAGAACGTTGAAAAAT | ACGCGTCGACTCACTCGCGAGGTCTAGCCAG |
| GhTGA3 | pCambia-CLuc | CGGGGTACCATGACAATATACGAGCAACTAAACC | ACGCGTCGACTCAGAGTGAACTGAGAGCTCGG |
| GhTGA4 | pCambia-CLuc | CGGGGTACCATGGCTAATGGGCTCATACTCCT | ACGCGTCGACTTATGCAGGTTCACGAGCACAAG |
| GhTGA5 | pCambia-CLuc | CGGGGTACCATGCCGGGTTTTGACTCACA | ACGCGTCGACTCACTCTCGGGGTCGGGC |
| GhBOP1 | pPZP111; gene-GFP | CGCGGATCCATGAGTAGCTTTGAGGAGTCCTTAAG | GTGGCCGCGGTAGAAATCATGAGAGTGATGGTACATT |
| GhTGA3 | pPZP111; gene-GFP | CGCGGATCCATGACAATATACGAGCAACTAAACC | GTGGCCGCGGCAGAGTGAACTGAGAGCTCGG |
| GhBOP1 | pMAL-p2X; MBP-GhBOP1 | CGCGGATCCATGAGTAGCTTTGAGGAGTCCTTAAG | CCCAAGCTTCTAGAAATCATGAGAGTGATGGTACATT |
| GhTGA3 | pGEX6P-1; GST-GhTGA3 | CGCGGATCCATGACAATATACGAGCAACTAAACC | CGCGAATTCTCAGAGTGAACTGAGAGCTCGG |
| ProGhPR1 | pBIN121 | CCCAAGCTTTCACATAGATTTGGTGGGTAGGG | CGCGGATCCTTTGAAGTTTGATTCTATAAGAATATTGC |
| GhTGA3 | pHANNIBAL-1; RNAi | CGGGAATTCCTGAGCGTTTCTTCCATTGGAT | CGGGGTACCGCTCGGAGACGGTTGAAGTAA |
| GhTGA3 | pHANNIBAL-2; RNAi | CGGTCTAGACTGAGCGTTTCTTCCATTGGAT | CGCGGATCCGCTCGGAGACGGTTGAAGTAA |
| GhBOP1 | pTL156; VIGS | CGGGGTACCCAGAAACAAACCACAGCACTCC | CGCGGATCCTAGGGTTGTCTTTCACGAGTAAA |
| GhBOP2 | pTL156; VIGS | CGGGGTACCCTAAAGTTTTCTCTTTTCTCTGTCTATC | CGCGGATCCGGTTGTTTTCCCAGAGGAATAC |
| GhBP1 | pTL156; VIGS | CGGGGTACCCGGGAAGAACTAACGAGGCC | CGCGGATCCGTTGATTCAGCCAATGCCACC |
| GhTGA3 | pTL156; VIGS | CGGGGTACCCTGAGCGTTTCTTCCATTGGAT | CGCGGATCCGCTCGGAGACGGTTGAAGTAA |
| NtHistone 3 | RT-PCR | TCCTGGGCAATTTCACGAACAAGC | TGCCCGTAAATCTACTGGAGGCAA |
| GhUB-7 | qPCR | GAAGGCATTCCACCTGACCAAC | CTTGACCTTCTTCTTCTTGTGCTTG |
| GhBOP1 | qPCR | AACCCACCAACTTCAACTGCGA | TCACCCTCCATTCTCGAACCCA |
| GhBOP2 | qPCR | AGAGCTTGGTGCAGCTGATGTT | TGGTGGTCTAAAAGCACCGCAA |
| ITS | qPCR | AAAGTTTTAATGGTTCGCTAAGA | CTTGGTCATTTAGAGGAAGTAA |
| GhPAL1 | qPCR | TCCAGGACAAATTGAGGCAGCG | CCAAGCCACTGTGGAGAAGTCC |
| GhC4H1 | qPCR | CCGAACCCGACACCCATAAGC | GCAGGGATGTCATACCCACCAAG |
| Gh4CL1 | qPCR | GTGTCTTGCCTTTATTCCACATTTAC | TTCTTAGCCAACAACACCACCAAC |
| GhC3H1 | qPCR | ACTATTATTGGACTTCTTTGGGACAT | ATCAGTTTCAGACATCACCCTTTC |
| GhCCoMT1 | qPCR | CACCTGGGTCACAATCCCTTAC | CCAACTGTGGCACGGCAAT |
| GhCAD5 | qPCR | CCGACCATGCGACTCAGACAAT | CTTGGGTGGGTTTTCCGTCAGT |
| GhPR1 | qPCR | GCTCTTGTAGGTGCTCTTGTTCTTCCCT | CTGGTTGTGAACCCTTAGATAATCTTGTGG |
| GhPR2 | qPCR | CAATCTCCCTTGCTCGTGAATCTCTACC | CGTTATCAACAGTGGACTGGGCGG |
| GhPR3 | qPCR | TTAACGGCCTCCTCGAAGCTGCTATTT | CGCAACATAAACAGTGAAACATCATTGGAA |
| GhPDF1.2 | qPCR | CAAGTGGGACATGGTCAGGGGTT | CACTTGTGTGCTGGGAAGACATAGTTGC |
| GhBP1 | qPCR | AGCGGCTCCAACGTGAGTAAT | GCTTCCAAGAGGTTAGAGTACTGAGG |
Solid lines under sequences indicate restriction enzyme cutting sites