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. 2019 Jun 21;9:9012. doi: 10.1038/s41598-019-45439-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Depth Scanning Correlation Enhancement Procedure: An image stack is captured by actuating the sample stage in a saw tooth pattern and cross correlation analysis is performed between the pixel intensity value and the actuation signal (position of the sample stage) for each pixel individually. The correlation image is composed of calculated cross-correlation values.