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. 2019 Jun 21;9:9022. doi: 10.1038/s41598-019-45440-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunization with rPfMSP2-based vaccines elicits high-titer PfMSP2-specific antibodies. New Zealand White (NZW) rabbits were immunized three times with rPfMSP2 (n = 4, black bars) or rPfMSP2/8 (n = 4, white bars) formulated with Montanide ISA 720. Antigen-specific IgG titers (mean ± SD) following each immunization were analyzed by ELISA. Signal detected by sera from the adjuvant control rabbit (n = 1) was subtracted from individual titers as background (Friedman test with Dunn’s multiple comparisons post hoc test; ns, not significant).