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. 2019 Jun 21;9:9022. doi: 10.1038/s41598-019-45440-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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rPfMSP2-based vaccines induce antibodies that recognize established mAb epitopes. To determine the impact of antigen conformation on antibodies induced by immunization, PfMSP2 peptides containing established mAb epitopes were used for analysis via ELISA. Sera from individual immunized rabbits (black, white) and control rabbit (grey) were diluted and tested for reactivity with each peptide. Data are presented as the average of duplicate A₄₀₅ values. Underlined portion of peptide sequence represents mAb epitopes 6D8, 11E1, 9D11 and 4D11/9G8 (left to right).