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. 2019 Jun 21;9:9022. doi: 10.1038/s41598-019-45440-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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rPfMSP2-associated fibrils mask relevant PfMSP2-specific epitopes. Competition ELISAs were conducted using a fixed concentration of rabbit antibody induced by (A) rPfMSP2 or (B) rPfMSP2/8 immunization. Sera were co-incubated in solution with increasing amounts of rPfMSP2 or rPfMSP2/8. Residual binding to plate-bound immunizing antigen for each antigen + antibody cocktail was measured in duplicate by ELISA and calculated as (A₄₀₅ of IgG with inhibitor/A₄₀₅ without inhibitor) × 100. Statistical significance was conducted at maximum levels of inhibition. Data from samples within an immunization group were averaged and compared. Asterisk indicates significance (Mann-Whitney U test, p < 0.03; ns, not significant).