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. 2019 Mar 20;76(14):2833–2850. doi: 10.1007/s00018-019-03070-z

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 5 — The effect of E2F1 on the expression of POLD1. 38PD 2BS and 35PD WI-38 cells were transfected with shRNA targeting E2F1 and E2F1 expression lentivirus. RT-PCR and western blotting were used to analyze the expression levels of E2F1 and POLD1. Control shRNA and pLenti-CMV were used as negative controls, and a bar graph indicates the relative expression levels of POLD1 and E2F1, normalized to GAPDH. The data were compared by one-way ANOVA, with three independent experiments in each group; *p < 0.05, **p < 0.01, ***p < 0.005, vs. the normal control (NC)