Fig. 7.

The variation in the binding affinity of E2F1 for the POLD1 promoter in replicative senescence and the effects of E2F1 and 5-AzaC treatment on the binding affinity of E2F1 for the POLD1 promoter in 2BS and WI-38 cells. Chromatin immunoprecipitation (ChIP) analysis was used to evaluate the binding affinity of E2F1 for the POLD1 promoter. a, b The binding affinity of E2F1 for the POLD1 promoter in different PDs of 2BS cells. c, d The binding affinity of E2F1 for the POLD1 promoter in different PDs of WI-38 cells. e, f The binding affinity of E2F1 for the POLD1 promoter in 48PD 2BS and 38PD WI-38 cells transfected with shRNA and expression lentivirus of E2F1. g, h The binding affinity of E2F1 for the POLD1 promoter in 48PD 2BS and 38PD WI-38 cells treated with 5 μM 5-AzaC after 72 h. i, j The binding affinity of E2F1 for the POLD1 promoter in 48PD 2BS and 38PD WI-38 cells treated with 5 μM 5-AzaC after 24 h and/or transfected with the expression lentivirus of E2F1. Densitometric analysis of the band intensity in different groups, normalized to the input. The data were compared by one-way ANOVA and the independent samples t-test, with three independent experiments in each group, *p < 0.05, **p < 0.01, ***p < 0.005, vs. the NC