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. 2019 Jun 21;9:9071. doi: 10.1038/s41598-019-45266-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of PP2C-37b, PP2C-77a, ABI5-5b, SAPK3, and HPt3a overexpression on the abiotic stress resistance of apple calli. (a) Apple calli transfected with an empty vector (1), PP2C-37b-overexpression calli (2), PP2C-77a-overexpression calli (3), ABI5-5b-overexpression calli (4), SAPK3-overexpression calli (5), and HPt3a-overexpression calli (6) were grown under 16 °C with 40% PEG8000 and 100 mM NaCl simultaneously. (b) Measurement of calli weight. Transfected apple calli were weighed before treatment (0 d) and after 12 d of treatment. (c) Relative intensity of NBT staining for transfected apple calli after treatment. (d) Relative intensity of DAB staining for transfected apple calli after treatment. Samples with different letters are significantly different P < 0.05.