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. 2019 Jun 21;9:9055. doi: 10.1038/s41598-019-45099-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of SCH on 3T3-L1 preadipocyte differentiation and lipogenesis. Measurement of relative lipid accumulation in maturated 3T3-L1 cells with spectrophotometric measurement of Oil Red O staining. Intracellular lipid accumulation and adipocyte maturity were investigated with microscopic analysis (B). Cells were untreated (a), treated with 10 μg/ml SCH (b) or treated with 50 μg/ml SCH (c). Images were taken at magnification of 200×. Quantitative real-time PCR showing the effect of SCH on gene expression involved in adipocyte differentiation and lipogenesis (C). All gene expression was normalized to β-actin gene expression to calculate relative expression. Data are expressed as mean ± SD.