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. 2019 Jun 21;9:9055. doi: 10.1038/s41598-019-45099-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western blot analysis showing the effect of SCH on phosphorylation of AMPK, ACC or protein expression related to energy metabolism and lipogenesis in FFA-induced steatotic HepG2 cells. (A) Representative blots of proteins are depicted. (B) Band intensity was measured with densitometric analysis and normalized to the intensity of non-phosphorylated AMPK or non-phosphorylated ACC or β-actin. Data are expressed as mean ± SD.