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. 2019 Jun 21;9:9060. doi: 10.1038/s41598-019-45515-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sox2-EGFP expression in prosensory cells of the embryonic cochlea. (a) Shows the tissue isolation and FACS pipeline used to generate the cells. (b–f) Show Sox2-EGFP expression (green) in vibratome sections of cochlea at the indicated stages of embryonic development. Sox2 immunofluorescence (magenta) demonstrates both the prosensory cells in the cochlear duct and the glia of the spiral ganglion. Note that Sox2-EGFP expression corresponds to Sox2 immunofluorescence and that Sox2-EGFP developmental dynamics mirror those of endogenous Sox2 expression. For example, the Sox2⁺/Sox2-EGFP⁺ field of cells in the floor of the cochlear duct narrows between E12.5-16.5—first at the base of the duct, then apically (compare b,d and f) and first at the lateral side of the duct, then medially (compare c, e and g). The localization of Sox2-EGFP expression shown here is representative of that in at least three temporal bones. Scale bars = 100 μm. sg, spiral ganglion; sa, saccule; ut, utricle.