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. 2019 Jun 21;9:9059. doi: 10.1038/s41598-019-45468-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Heat shock induces yb-1 transcript levels in zebrafish but not in mammalian cells. (a) cell viability of PAC2 cells after heat shock treatment at 45 °C. Cells were subjected to elevated temperatures for the times indicated on the x-axis and cell viability values (MTT) are plotted on the y-axis. Statistical analysis was performed using 1-way ANOVA followed by Dunnett’s multiple comparisons test; ns indicates no statistical significance (see also Table S1); (b–d) RT-qPCR analysis of zebrafish zf hsp70 (b), zf yb-1 (c) in PAC2 cells and human h yb-1 (d) in HaCaT cells. Samples were taken at different time points during and after 1 hour of heat shock treatment at the indicated temperatures (for precise experimental details, see materials and methods section). Mean mRNA relative expression (n = 3) ± SD is plotted on the y-axes, whereas time is plotted on the x-axes. Statistical analysis was performed using 2-way ANOVA and Sidak’s multiple comparison test. Levels of significance between points of expression and time 0 are indicated (***p < 0.001, **p < 0.01, *p < 0.05) (see also Table S1 for statistical analysis).