Figure 2.

Ligand-binding specificity of the Tiam2 QM PDZ domain. (A) Representative binding curves of the Tiam2 QM PDZ. The K_d values reflect the mean and SD from at least three technical replicates. (B) Shown is a summary of the peptide-binding data for Tiam2 WT, Tiam2 QM, and Tiam1 WT PDZ domains. The color coding shows graphically the trend in affinity of the Tiam2 QM and Tiam1 WT compared to the Tiam2 WT PDZ. The column labeled “Trend” indicates with an arrow the trend in affinity (higher (↑) or lower (↓)). The scale of the color coding is defined at the bottom. To see this figure in color, go online.