Structural changes of WT and mutants of the Tiam2 PDZ domain. Shown is an overlay of 1H-15N HSQC spectra of WT Tiam2 PDZ with Tiam2 PDZ mutants: Tiam2 PDZ E979K (A), Tiam2 PDZ F982L (B), Tiam2 PDZ E979K/F982L (C), Tiam2 PDZ M978L/V987L (D), and Tiam2 PDZ M978L/E979K/F982L/V987L (QM) (E). Select residues with perturbed chemical shifts are labeled. To see this figure in color, go online.