Table 4.
Coupling Free Energies of Folding for Tiam2 PDZ Mutants
| Tiam2 PDZ Domain | Cm [mM]a | ΔGf (kcal/mol) | ΔGint (kcal/mol)b |
|---|---|---|---|
| WT | 1.26 ± 0.02 | −3.08 ± 0.18 | |
| E979K | 1.29 ± 0.01 | −3.19 ± 0.16 | |
| F982L | 1.33 ± 0.02 | −3.27 ± 0.15 | |
| E979K/F982L | 1.41 ± 0.02 | −3.91 ± 0.17 | −0.53 ± 0.33 |
| M978L/V987L | 1.60 ± 0.02 | −4.46 ± 0.17 | |
| QM | 1.78 ± 0.03 | −5.04 ± 0.17 |
a
Abbreviations: Cm, concentration of Gdn-HCl at ΔGf = 0; f, folding; int, interaction; QM, quadruple mutant (M978L/E979K/F982L/V987L).
b
ΔGint = [ΔGf (WT) − ΔGf (mutant1)] − [ΔGf (mutant2) − ΔGf (mutant1,2)]. The error of individual measurements was estimated from triplicate measurements and propagated to obtain the error in coupling free energy.