Table 1.
Summary of the final three decellularization protocols and subsequent sterilization procedure that were optimized in this study. Treatments below the dotted line were equal to all protocols
| Details | Protocol 1 | Protocol 2 | Protocol 3 |
|---|---|---|---|
| SDS | SDC | SDS + SDC | |
| Detergent 1 | 0.5% SDS (10 h) | 2% SDC (16 h) | 0.5% SDS (5 h) |
| Wash | dH2O (24 h) | dH2O (24 h) | dH2O (15 h) |
| Detergent 2 | – | – | 2% SDC (8 h) |
| Wash | – | – | dH2O (24 h) |
| Pre-treatment | PBS (1 h) | ||
| Enzyme | 40 IU DNase / ml of PBS (30 min; 37 °C) | ||
| Wash | PBS (24 h) | ||
| Sterilization | 0.1% Peracetic acid in normal saline (30 min) | ||
| Wash | Sterile PBS (6 × 5 min) | ||
| Wash | Sterile PBS (24 h) | ||
| Total time | 85 h | 91 h | 103 h |
Abbreviations: SDS sodium dodecyl sulfate, SDC sodium deoxychocolate, dH2O deionized water, PBS phosphate buffered saline, h hours, min minutes