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. 2019 Jun 20;87(7):e00080-19. doi: 10.1128/IAI.00080-19

FIG 6.

FIG 6

OG1RF_12399-12402 increase intramacrophage E. faecalis survival and proinflammatory cytokine secretion in vitro and bacterial translocation to mesenteric lymph nodes (MLN) in vivo. (A and B) Fraction of gentamicin-resistant (i.e., intracellular) E. faecalis bacteria in Caco-2 human intestinal epithelial cells (A) and J774A.1 mouse monocyte-macrophage cells (B). (C and D) IL-12/23p40 (C) and TNF-α (D) secretion by E. faecalis-infected J774A.1 cells referenced in panel B. (E) CFU per mesenteric lymph node cell in wild-type mice monocolonized for 2 weeks with either E. faecalis OG1RF or E. faecalis OG1RFΔ12399-12402. Data are presented as means ± SEM (n = 3 wells/bacterial strain/time point or 3 to 5 mice/group). Data in panels A and B are expressed as a percentage of intracellular bacteria at 1 h postinfection. *, P < 0.05 by Student’s t test.