FIG 1.

SDS-PAGE of the binding assays between Tet38-His and CD36 or TLR-2. Enterokinase-treated CD36 or enterokinase-treated TLR-2 was applied to an Ni column that had been previously loaded with Tet38-His. Flowthrough (FT), wash, and elution fractions were collected and submitted to SDS-PAGE (top middle and right). Reference proteins included Tet38, CD36, and TLR-2 (top left). In the cases of CD36 and TLR-2, enterokinase-treated CD36 or TLR-2 was applied to an Ni column (bottom left and middle). For NorA-His plus CD36, purified NorA-His protein was loaded onto an Ni column and used to retain CD36 as was done with Tet38 (bottom right). Reference proteins included CD36 and NorA (bottom right).