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. 2019 Jun 20;87(7):e00194-19. doi: 10.1128/IAI.00194-19

FIG 2.

FIG 2

SDS-PAGE of binding assays between CD36 and TLR-2. Enterokinase-treated TLR-2 was applied to an Ni column that had been previously loaded with CD36-His. CD36 and TLR-2 proteins were found in the flowthrough fraction, absent in the wash fraction, and then were again eluted together by 100 mM imidazole (middle). Enterokinase-treated CD36 was applied to an Ni column that had been previously loaded with TLR-2-His. CD36 protein was found in the flowthrough fraction, absent in the wash fraction, and eluted together with TLR-2 by 100 mM imidazole (right). Reference proteins included CD36 and TLR-2 (left).