FIG 3.

SDS-PAGE and Western blots of binding assays with purified Tet38-His, CD36, and TLR-2. CD36 and then TLR-2 (both enterokinase treated) were applied to an Ni column that had been previously loaded with Tet38-His. CD36 and TLR-2 were found in the flowthrough and elution fractions but not in the wash fraction (top right). Reference proteins included Tet38, CD36, and TLR-2 (top left). Reference proteins (bottom left) and proteins recovered in the elution fractions (bottom right) were submitted to Western blotting using anti-His (for Tet38-His), anti-CD36 (for CD36), and anti-TLR-2 (for TLR-2) antibodies separately to verify their presence in the elution fraction.