FIG 4.

SDS-PAGE of the binding assays between Tet38-His, CD36, and TLR-2, with or without LTA. Enterokinase-treated CD36 and TLR-2 were applied successively to an Ni column that had been previously loaded with Tet38-His. TLR-2 and CD36 were found in the flowthrough fraction, and CD36, TLR-2, and Tet38-His were eluted together by 100 mM imidazole (third image). Enterokinase-treated CD36, TLR-2, and LTA were applied successively to an Ni column that had been previously loaded with Tet38-His. TLR-2, CD36, and LTA were found in the flowthrough. CD36 and Tet38 were eluted together by 100 mM imidazole (fourth image). As a control, LTA was applied to an Ni column that had been previously loaded with TLR-2-His. TLR-2-His and LTA were found in the flowthrough fraction, and they were eluted together by 100 mM imidazole (second image).