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. 2019 Jun 20;87(7):e00194-19. doi: 10.1128/IAI.00194-19

FIG 5.

FIG 5

SDS-PAGE of the binding assays between CD36-His and TLR-2 with or without LTA. Enterokinase-treated TLR-2 was applied to an Ni column that had been previously loaded with CD36-His. TLR-2 and CD36-His were found in the flowthrough fraction, and again were eluted together by 100 mM imidazole (middle panel). Enterokinase-treated TLR-2 and LTA were applied successively to an Ni column that had been previously loaded with protein CD36-His. TLR-2, CD36, and LTA were found in the flowthrough fraction and then again were eluted together by 100 mM imidazole (right).