TABLE 1.
Internalization of S. aureus by A549 cellsa
| S. aureus | Bacterial CFU/well |
|||
|---|---|---|---|---|
| Nonspecific IgG | +LTA antibodyb | +CD36 antibodyb | +TLR-2 antibodyb | |
| RN6390 | 1,300 ± 100 | 400 ± 25 | 400 ± 30 | 450 ± 25 |
| QT7 | 240 ± 15 | 110 ± 15 | 200 ± 10 | 120 ± 30 |
| QT5 | 1,400 ± 150 | 450 ± 50 | 400 ± 50 | 480 ± 30 |
a
All values represent the means from three independent experiments. Values represent the differences between the CFU of RN6390 and QT7 in the presence of antibodies and are statistically significant based on Student’s t test (P < 0.05), or values represent the differences between the CFU of QT7 in the presence of nonspecific IgG antibody versus the CFU of QT7 in the presence of anti-LTA and anti-TLR-2 antibodies and are statistically significant based on Student’s t test (P < 0.05). QT7 is a tet38 mutant; QT5 is a norB efllux pump mutant.
b
Concentrations of LTA, CD36, and TLR-2 antibodies are 50 nM per well.