TABLE 2.
Effect of purified Tet38 protein on the internalization of S. aureus RN6390 by A549 cells
| Condition |
S. aureus RN6390 (CFU/well)a
|
|
|---|---|---|
| +Nonspecific IgG | +CD36 antibody | |
| No purified proteins | 1,540 ± 100 | 450 ± 25 |
| Purified Tet38 | 1,499 ± 95 | 700 ± 50 |
| Purified NorA | 1,480 ± 80 | 440 ± 30 |
a
All values represent the means from three independent experiments. Values represent the differences between the CFU of RN6390 in the presence of anti-CD36 antibody, with and without purified Tet38 proteins, and are statistically significant based on Student’s t test (P < 0.05). Concentrations of IgG and CD36 antibodies are 50 nM per well. Proteins (Tet38 or NorA) were used at 5 μg per assay.