FIG 6.
Effect of IcsP-mediated cleavage of IcsA on biofilm formation. (A) xy, yz, and xz views of the biofilms formed by the WT and icsP mutant strains. (B) Quantification of biofilm thickness. Symbols in the graph represent the average biofilm thicknesses in 25 fields of view for each biological replicate. Unpaired Student’s t test was applied for statistical analysis using GraphPad Prism 7.02. *, P value of <0.05. Error bars indicate the standard errors of the means from three biological replicates. (C) IcsA-Myc is not cleaved in the icsP mutant background. Shown are representative data from Western blot analysis of IcsA-Myc species in the biofilms formed by the WT/pIcsA-Myc (WTIcsA-Myc) and icsP/pIcsA-Myc (icsPIcsA-Myc) strains in TSB-BS.