TABLE 3.
Primers used in the study
| DNA manipulation and primer name | Sequence (5′→3′)a |
|---|---|
| S2406 deletion | |
| 5S2406 | TGAGATCCAGCAAGATCACGTCG |
| 3S2406 | CTAAGGAGGATATTCATATGTTGATGCATTGAGCTTTCATCCTATG |
| 5S2406-CAT | CATAGGATGAAAGCTCAATGCATCAACATATGAATATCCTCCTTAG |
| 3S2406-CAT | CTGTTATCAGAACGTCCAGACCACGTGTAGGCTGGAGCTGCTTC |
| 5CAT-S2406 | GAAGCAGCTCCAGCCTACACGTGGTCTGGACGTTCTGATAACAG |
| 3CAT-S2406 | CGCAAGGTCAGGATTTGTTCAACC |
| S1242 deletion | |
| 5S1242_NotI | AATTGCGGCCGCGACAGGATTGCCATTAGTAGCATG |
| 3S1242_blunt | AATTCATTGCGTTAACAGTCAATGACAC |
| 5S1242-CAT | GCAGGGTAAAAACACGATTGTCACCATATGAATATCCTCCTTAG |
| 3S1242-CAT | CTAAGGAGGATATTCATATGGTGACAATCGTGTTTTTACCCTGC |
| 5CAT-S1242 | CGAAGCAGCTCCAGCCTACACGGTGGGCCTGAAATATAAGTTC |
| 3CAT-S1242 | GAACTTATATTTCAGGCCCACCGTGTAGGCTGGAGCTGCTTCG |
| icsA deletion | |
| 5icsA-UP-SacI | AATTGAGCTCCAGGGCGTGTTGATGTCCTGC |
| 3icsA-UP-XhoI | AATTCTCGAGGTAAGTGGTTGATAAACCCCTG |
| 5icsA-DOWN-SacI | AATTGAGCTCCAGGGCGTGTTGATGTCCTGC |
| 3icsA-DOWN-BamHI | AATTGGATCCCGACCCCGTACTGCTCACGCA |
| S1289 deletion | |
| 5S1289-UP-NotI | AATTGCGGCCGCGGCTTTATCCGTGCACAAACC |
| 3S1289-UP-XhoI | AATTCTCGAGCCACTCCTATATAGTACCCAGG |
| 5S1289-DOWN-SacI | AATTGAGCTCCAACGGAGGATATCGCTTCAGC |
| 3S1289-DOWN-PstI | AATTCTGCAGCCTGTCCTGCTTTGTGGAGAAG |
| icsP deletion | |
| 5icsP-UP-NotI | ATATGCGGCCGCGATTAGTCCTTAATCGGACAACCAC |
| 3icsP-UP-XhoI | ATATCTCGAGGATCTTCTTACTTTATAAGATAAACGTCAA |
| 5icsP-DOWN-SacI | GGGGAGCTCATTGGTTCACCGAGTTGATTACGTT |
| 3icsP-DOWN-PstI | GGGCTGCAGGTCCCTGATAGCACTGTTCCATCA |
| IcsA expression | |
| FW-SmaI | ATTACCCGGGGGTTAGTTATGTTTGATGTCTGC |
| REV-SalI | ATTAGTCGACCCTCGTACAGAACTACTCAAGTCC |
| IcsA-Myc expression | |
| 5IcsA-UP-BplI | GGCCATGCGTCTGGTATTACC |
| 3IcsA-Myc-748aa | AAGATCTTCTTCAGAAATAAGTTTTTGTTCGCTCATCTGTTTTGATTCTTGA |
| 5IcsA-Myc-748aa | GAACAAAAACTTATTTCTGAAGAAGATCTTAATCAAGAGTCTACTCAAAT |
| 3IcsA-DOWN-BbsI | CCATAAGCTCCCATAATACCC |
a
Restriction sites are in boldface type.