Figure 2. Mortality, heart rate and hatching success in percentage of unbuffered and pH 7 treatment.

(A) Mortality after 96 hpf (likelihood ratio χ², Fisher’s exact test, Bonferroni-Holm, p < α), (B) heart rate at 48 hpf relative to the negative control (Steel-Dwass, p < 0.01), (C) hatching rate over time in unbuffered treatments (Cox regression, p < 0.05), (D) hatching rate in pH 7 treatments over time (Cox regression, p < 0.001); shaded bars mark treatments with n < 5 that show tendencies but are not included in the statistical analyses.