Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 3;132(11):jcs227033. doi: 10.1242/jcs.227033

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 1. — DnaJB6 is a RanGTP-regulated protein associated to the mitotic spindle. (A) Immunofluorescence on HeLa cells transfected with Flag-tagged long and short DnaJB6 isoform-expressing constructs. DnaJB6-L (long) localizes in the nucleus during interphase and to the spindle poles in mitosis. DnaJB6-S (short) shows diffuse cytoplasmic localization both during interphase and mitosis. (B) Western blot analysis of a GST–xDnaJB6-L pulldown experiment in Xenopus laevis egg extract. Importin-β associates with GST–xDnaJB6-L and is released by addition of RanGTP to the extract (top). The lower panel shows that similar levels of GST–xDnaJB6 were used for pull downs in the presence or absence of RanGTP. (C) Amino acid sequence alignment of the putative NLS in human (top) and Xenopus laevis (bottom) DnaJB6 long isoforms. Asterisks highlight identical amino acids. (D) Immunofluorescence images of HeLa cells showing the localization of DnaJB6 in different cell cycle stages using an in-house generated antibody. DnaJB6 accumulates in the nucleus during interphase and localizes to the spindle during mitosis with an accumulation at the spindle poles in metaphase. Tubulin is shown in red, DnaJB6 in green and DNA in blue. Scale bars: 10 μm.