Figure 1. The optoPAD system.

(A) Concept for the use of closed-loop capacitance measurement of feeding with optogenetic manipulation of neurons in behaving flies. The interaction of the fly with the food source triggers the activation of the LED. (B) Overview of the components of the optoPAD, the flyPAD arena and the high-power RGBA LEDs. (C) Algorithm for real-time detection of food interactions. Extracted food interaction bouts (activity bouts) are shaded in gray. (D) Schematic overview of the optoPAD experimental dataflow.

Figure 1—figure supplement 1. Quantification of the probability of the occurrence of a feeding burst onset depending on the time after activity bout onset.

Bins are 0.1 s wide and probabilities are plotted for two different genetic background controls feeding on yeast, on an optoPAD channel triggering light activation.