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. Author manuscript; available in PMC: 2020 Apr 15.
Published in final edited form as: Methods. 2019 Jan 24;159-160:129–137. doi: 10.1016/j.ymeth.2019.01.010

Fig. 2.

Fig. 2.

Design of pUASTattB-Rpb1 and cross schemes to generate stable fly lines expressing various GAL4 and UAS transgenes. Blue genotypes designate steps where meiotic recombination events occur. In Drosophila, meiotic recombination is limited to females. Offspring with TM3 third chromosome balancer can be distinguished by their short bristles (Sb1). Primers for verifying the presence of pUASTattB-Rpb1 in the fly genome: fw: 5’-CGCCTTCGGCTGCATCGG-3’ and rev: 5’-ACAAAGATCCTCTAGAGGTACCCTCGAGC-3’. Primers for detecting UAS-Rpb1i transgene: fw: 5’-GGTGATAGAGCCTGAACCAG-3’ and rev: 5’-TAATCGTGTGTGATGCCTACC-3’. Primers for detecting da-GAL4 transgene: fw: 5’-GGATGCTCTTCATGGATTTG-3’ and 5’-CAACATCATTAGCGTCGGTGAG-3’.