Skip to main content
. Author manuscript; available in PMC: 2020 Apr 15.
Published in final edited form as: Methods. 2019 Jan 24;159-160:129–137. doi: 10.1016/j.ymeth.2019.01.010

Fig. 6.

Fig. 6.

Alternative detection of the modified endogenous Rpb1 locus by PCR. (A) Schematic of PCR validation of the tagged CTD fly lines. Magenta arrows represent primers. One of the primers hybridizes to the acidic tip (red) that is retained in CTD mutants. The other primer hybridizes to the 3’ UTR of Rpb1. Sequences of primers: fw: 5’-TCATACAGTGGGTTGTGCAAAGAA-3’ and rev: 5’-ACGTTCGAGGAGAGCGAAGAC −3’. (B) PCR reactions with primers annotated in (A). Genomic DNA was isolated from one male fly [34]. The increased sizes of the PCR products with HA-Rpb1 and FLAG-Rpb1 compared with w1118 suggested that the desired tags were introduced.