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. Author manuscript; available in PMC: 2019 Jun 23.
Published in final edited form as: Biochem J. 2012 Feb 15;442(1):95–103. doi: 10.1042/BJ20111145

Figure 6. Binding of WT- and A13T-reconstituted porcine myosin to pyrene-labelled F-actin under rigor conditions.

Figure 6

Association of myosin with actin resulted in quenching of pyrene fluorescence. The binding isotherms were fitted to a non-linear binding model as explained in the Materials and methods section. Inset: SDS/PAGE of RLC-depleted myosin (lane 2), used for reconstitution (reconst.) with the recombinant WT and A13T-RLC proteins (lanes 3 and 4). Lane 1 depicts native porcine myosin.